Figure 1

Growth inhibition of gastric cancer cells by MLN4924. (a) MLN4924 inhibited the neddylation of CUL1. AGS and MGC80-3 cells were treated under the indicated MLN4924 concentrations for 24–72 h, and then subjected to immunoblotting analysis of neddylation of CUL1. (b) MLN4924 suppressed the proliferation of gastric cancer cells. AGS and MGC80-3 cells were treated under the indicated MLN4924 concentrations for 24–96 h, then trypsinized and counted at each time point. (c) MLN4924 impaired the viability of gastric cancer cells. AGS and MGC80-3 cells were treated under the indicated MLN4924 concentrations and subjected to CCK8 colorimetric assay to assess their viability. (d) MLN4924 affected the colony-formation ability of gastric cancer cells. AGS and MGC80-3 cells were treated under the indicated MLN4924 concentrations for 9 days and then subjected to colony-formation assay. For panels b and c, n=3, and for panel d, n=4