Figure 5
From: The novel protective role of P27 in MLN4924-treated gastric cancer cells
ROS overproduction following combination treatment resulted in augmented apoptosis. AGS and MGC80-3 cells transfected with NC or siP27 were treated under the indicated combination of MLN4924 and antioxidants. (a) The siP27-induced apoptosis augmentation in MLN4924-treated gastric cancer cells. The apoptosis was determined by Annexin V/propidium iodide double staining and FCM. (b) P27 silencing exacerbated ROS overproduction in MLN4924-treated cells. ROS levels were quantified by DCFH-DA staining and FCM. For panel a and b, n=3
