Figure 7
From: The novel protective role of P27 in MLN4924-treated gastric cancer cells
ROS triggered the collapse of MMP via siP27-induced profile alteration of BCL-2 family members in MLN4924-treated cells. (a) Deterioration of MMP collapse in the MLN4924-treated cells expressing low P27 was prevented by antioxidants. AGS and MGC80-3 cells transfected with NC or siP27 were treated under the combination of MLN4924 and antioxidants for 72 h, and subjected to JC-1 staining and FCM for the determination of MMP collapse. (b) P27 silencing led to alteration of BCL-2 family members expression in MLN4924-treated cells. AGS and MGC80-3 cells transfected with NC or siP27 were treated with 0.3 μM MLN4924 for 72 h, and subjected to immunoblotting analysis for the detection of expression and status of indicated proteins. For panel a, n=3
