Figure 4 | Cell Death & Disease

Figure 4

From: Neuritin 1 promotes retinal ganglion cell survival and axonal regeneration following optic nerve crush

Figure 4

Increased expression of hNRN1 in vivo after intravitreal injection of AAV2-CAG–hNRN1. BALB/cJ mice received intravitreal injections of AAV2–hNRN1 and were harvested at naïve, 2 and 3 weeks after injection. Fluorescence micrographs show overexpression of hNRN1 in longitudinal (a) retinal, (b) retina and ON and (c and d) SC coronal sections. Green=NRN1; red=Brn3a; blue=DAPI. Time point post injection indicated on top of the panel. RSC (right contralateral superior colliculus) and LSC (left ipsilateral superior colliculus). Scale bar=50 μm (a), 100 μm (b–d), n=4. Photomicrographs were captured at 400 × and 100 × original magnification

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