Figure 5
AAV2-mediated delivery of NRN1 in vivo promoted and sustained survival of axotomized RGCs. Animals were intravitreally injected with either AAV2–GFP or AAV2–hNRN1. Two weeks later, animals were subjected to ONC and whole eyes harvested at 7, 14, 21 and 28 days post crush. (a) Representation of retinal tissue sections counted from ora serrata to ora serrata in the central region of each mouse retina (six sections/eye). Fluorescent micrographs of Rbpms and Rbpms with DAPI images at 14 days post crush show representative images captured at each point throughout the whole retina to count the Rbpms-positive cells (400 × magnification, Scale bar=50 μm). (b) Quantification of Rbpms-positive cells. Naïve retinas presented a mean of 168±23 cells (n=4). Data are presented as mean±S.E.M. Statistical significance between treatment conditions determined by unpaired Student’s t-test, *P<0.05, **P<0.01, n=3–7
