Figure 2
From: BAC transgenic mice provide evidence that p53 expression is highly regulated in vivo
Preferential p53 reporter expression in the proliferating compartments during mouse postnatal development, tissue homeostasis, regeneration and tumorigenesis. (a) β-gal staining of tissues including spleen, testis, dentate gyrus, pancreas, choroid plexus, cerebellum, kidney, lung and liver at P7, 2 months and 15 months of age respectively in PZU mice. Frozen slices of tissues were stained in 1 mg/ml X-gal staining solution at 37 °C for 10 h, except for 3 h in testis. Scale bar=200 μm. (b) β-gal staining of skins at the anagen (p20) or telogen (p24) phase of the hair cycle in PZU mice. Frozen slices of skins were stained in 1 mg/ml X-gal staining solution at 37 °C for 10 h. Boxed areas were shown in the right panel. Scale bar=200 μm. (c) Double immunofluorescence of β-gal and Ki67 in p8 cerebellum and adult small intestine of PZU mice. Scale bar=200 μm. (d) β-gal staining of liver before and 48 h after hepatotectomy. Frozen slices of livers were stained in 1 mg/ml X-gal staining solution at 37 °C for 7 h in PZU mice. Boxed areas were shown in the right panel. Scale bar=200μm. (e) β-gal staining of small intestine in PZU mice and polyps in PZU; Apcmin mice
