Figure 6
From: BAC transgenic mice provide evidence that p53 expression is highly regulated in vivo
A direct link between cellular proliferation and p53-mediated stress response. (a) IHC of p53 in small intestine, cerebellum and tongue of p7 and 2-month-old C57BL/6J control mice or mice treated with X-irradiation for 4 h. Arrows depicted positive stained cells. Scale bar=200 μm. (b) Double immunostaining of p53 and BrdU in intestine and testis of 2-month-old C57BL/6J mice treated with X-irradiation for 4 h. (c) Western blot analysis of p53 and p21 expression on quiescent and proliferating 3T3 cells treated with Doxorubicin. (d) Western blot analysis of p53 and p21 expression on quiescent and proliferating MEFs treated with Cisplatin. (e) Immunofluorescence of p53 or p21 in quiescent and proliferating MEFs treated with Cisplatin or Doxorubicin. Scale bar=200 μm
