Figure 1 | Cell Death & Disease

Figure 1

From: Bone marrow-derived mesenchymal stem cells ameliorate chronic high glucose-induced β-cell injury through modulation of autophagy

Figure 1

BM-MSCs protected INS-1 cells against chronic HG-induced injury. (a) Cellular viability was determined by CCK-8 assay. The data are expressed as percentages of untreated control cells. (b and c) Western blot analysis of cleaved caspase 3. Protein expression levels were normalized against β-actin. (d and e) Apoptosis incidence in INS-1 cells was detected by Annexin V-FITC/PI double staining. Cells at early stages of apoptosis were Annexin V-FITC positive but PI negative. Cells at late stage of apoptosis were both Annexin V-FITC and PI positive. These two stages combined measurements were counted as total apoptotic cells. (f) Insulin secretion levels at basal (5 mmol/l) or stimulatory (25 mmol/l) concentrations of glucose were determined using an insulin RIA kit and normalized to the respective protein contents. Results are means±S.E. of five independent experiments. *P<0.05, **P<0.01 versus control group; #P<0.05, ##P<0.01 versus HG group

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