Figure 4 | Cell Death & Disease

Figure 4

From: Bone marrow-derived mesenchymal stem cells ameliorate chronic high glucose-induced β-cell injury through modulation of autophagy

Figure 4

Inhibition of autophagy diminished the protective effects of BM-MSCs on HG-treated INS-1 cells. INS-1 cells co-cultured with BM-MSCs were treated with 3-MA (10 nM) and CQ (20 μM). (a and b) 3-MA reduced LC3-II expression by inhibiting class III PI3K at an early stage of autophagy; CQ led to additional accumulation of LC3-II by inhibiting lysosome protease at a late stage of autophagy. 3-MA and CQ treatment both resulted in a significant reduction of cell viability (d), upregulated expression of cleaved caspase 3 (a and c), increased incidence of apoptosis (e and f) and impaired BIS and GSIS (g). Results are means±S.E. of five separate experiments. *P<0.05, **P<0.01 versus control group; #P<0.05, ##P<0.01 versus HG group; #P<0.05, ##P<0.01 versus HG+BM-MSCs group

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