Figure 5 | Cell Death & Disease

Figure 5

From: Caspase-8 activation by TRAIL monotherapy predicts responses to IAPi and TRAIL combination treatment in breast cancer cell lines

Figure 5

Demonstrating Caspase-8 activity assay as a predictive biomarker feasibility in spheroid cultures. Spheroids of MDA-MB-361 (AZD5582+TRAIL combination resistant) and MDA-MB-436 (combination sensitive) cells were treated with 30 ng/ml TRAIL followed by the Caspase-8 activity assay 16 h later (a) or treated with 10 ng/ml TRAIL, 10 nM AZD5582 or a combination for 24 h followed by fluorescence detection of Caspase3/7 activity. (b) Xenograft-derived spheroids of MDA-MB-361 (AZD5582+TRAIL combination resistant) and MDA-MB-436 (combination sensitive) models were treated with 30 ng/ml TRAIL followed by the Caspase-8 activity assay 16 h later (c) or treated with 10 ng/ml TRAIL, 10 nM AZD5582 or a combination for 24 h followed by fluorescence detection of Caspase-3/7 activity. (d) Error bars represent S.E.M. of means of three independent experiments (a) or independent experiments performed on tumour-derived spheroids from three different mice (c). Images in (b) and (d) were obtained using lightsheet microscopy and presented as maximum intensity projections of 200 μm depth of the spheroid, Hoechst 33342 is blue, and Caspase3/7 signal is green. Scale bar represents 100 μm

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