Figure 1 | Cell Death & Disease

Figure 1

From: Smac mimetic-induced upregulation of interferon-β sensitizes glioblastoma to temozolomide-induced cell death

Figure 1

BV6/TMZ cotreatment upregulates IFN-responsive genes. (a) A172 cells (left) or T98G cells (right) were treated for indicated times with 100 μM TMZ and/or 2 μM BV6 (A172) or 4 μM BV6 (T98G) or dimethyl sulfoxide (DMSO). Apoptosis was determined by fluorescence-activated cell sorting (FACS) analysis of DNA fragmentation of PI-stained nuclei. Mean values +S.D. of three to four independent experiments performed in triplicate are shown; *P<0.05; **P<0.01 compared with all other settings. (b) A172 cells (left) or T98G cells (right) stably expressing IκBα-SR or EV were treated for 120 h with 100 μM TMZ and/or 2 μM BV6 (A172) or 4 μM BV6 (T98G) or DMSO. Apoptosis was determined by FACS analysis of DNA fragmentation of PI-stained nuclei. Mean values +S.D. of three independent experiments performed in triplicate are shown; *P<0.05; **P<0.01 compared with all other settings. (c) A172 cells (left) or T98G cells (right) stably expressing IκBα-SR or EV were analyzed for IκBα expression levels by western blotting. Expression of β-actin served as a loading control. A representative experiment of two independent experiments is shown

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