Figure 6 | Cell Death & Disease

Figure 6

From: Activation of RARα induces autophagy in SKBR3 breast cancer cells and depletion of key autophagy genes enhances ATRA toxicity

Figure 6

Inhibition of autophagy impacts on ATRA-induced epithelial differentiation of SKBR3 cells. (a) SKBR3 cells were treated for 4 days with 1 μM ATRA, increasing concentrations of chloroquine or a combination thereof as indicated before western blot analysis for VE-cadherin, β-catenin, LC3B and GAPDH. (b) Quantification of β-catenin and VE-cadherin western blots as one is shown in (a). Raw values were normalized to GAPDH and levels of ATRA-treated control transduced cells were arbitrary set to 100% as signals were not always detectable in vehicle-treated control cells. Bars and s.d. representative of five independent experiments. (c) SKBR3 control and ATG7-depleted cells were subjected to 1 μM ATRA for 4 days and expression levels of VE-cadherin, β-catenin, ATG7 and GAPDH were determined by western blot. (d) Quantification of western blots for β-catenin and VE-cadherin. Normalization was performed as described in (b)

Back to article page