Figure 3
Pref-1 protein and mRNA production is compromised in Tgm2−/− MEFs. (a) Western blot analysis of Pref-1f in total cell lysates of Tgm2−/− and Tgm2+/+ MEFs during early differentiation on days 0–3 show almost complete absence of the protein. Actin was used as loading control. (b) mRNA expression of Pref-1 by RT-PCR in the cells shows that on day 0 Pref-1 mRNA is lower in Tgm2−/− compared with Tgm2+/+ MEFs; however, on day 3 the difference is no longer observed. This mRNA does not appear to translate into protein as per Western blot analysis. Gapdh used as loading control. (c) Quantification of mRNA expression of Pref-1 (b), shows significantly reduced of Pref-1 on day 0 in Tgm2−/− compared with Tgm2+/+ MEFs. Pref-1 expression was similar in both Tgm2−/− and Tgm2+/+ MEFs after initiation of differentiation (day 1). Error bars±SEM (n=3), **P<0.01
