Figure 7
Tgm2−/− MEFs display reduced ROCK kinase activity, actin fibers and increased Akt phosphorylation. (a) Microplate ROCK kinase activity of Tgm2+/+ and Tgm2−/− MEFs in total cell lysate during differentiation show moderate but significant decrease in Tgm2−/− cells on days 1 and 2. Error bars±SEM (n=3), *P<0.05. (b) Immunofluorescence staining of of Tgm2+/+ and Tgm2−/− MEFs for F-actin on days 0 and 3. A moderate decrease in actin stress fibers is observed. Nuclei are stained with DAPI (blue). Scale bar 200 μm. (c, d) Western blot analysis and quantification of pAkt (Ser473) and total Akt in MEF cell lysates from day 0–3. An increase in Akt phosphorylation is seen on day 3 in Tgm2−/− MEFs compared with Tgm2+/+ MEFs. Results are mean values±SEM (n=3), *P<0.05
