Figure 5 | Cell Death & Disease

Figure 5

From: Inhibition of Notch1 signaling overcomes resistance to the death ligand Trail by specificity protein 1-dependent upregulation of death receptor 5

Figure 5

JNK is involved in Notch1-mediated DR5 regulation. (a) JNK is activated following Notch1 knockdown. Immunoblot analysis of Notch1 and phospho-JNK in cytoplasmic lysates of control-AdV and Notch1sh-AdV transduced U251MG cells 72 h post transduction. (b) U251MG cells transduced with control-AdV or Notch1sh-AdV (48 h) were treated with SP600125 (20 μM) for 24 h. Immunoblot analysis for Notch1, DR5, phospho c-jun and c-jun (left panel). Quantitative real-time PCR analysis of Notch1 and DR5 mRNA expression (right panel). Expression data were normalized to internal 18 S rRNA expression (n=3, mean±S.D.). P-values were determined by t-test (control-AdV versus Notch1sh-AdV) or two-way ANOVA (Notch1sh-AdV w/o SP600125 versus Notch1sh-AdV 20 μM SP600125)

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