Figure 3
From: Sonic hedgehog through Gli2 and Gli3 is required for the proper development of placental labyrinth
Expression patterns of the main components of Shh pathway in placentas (E12.0) after genetic modifications. (a–f’ and s) Shh+/− and Shh−/− placentas, (g–l’, and t) Shh−/− placentas infected with either control-shRNA- or Gli3-shRNA-expressing lentiviruses, and (m–r’ and u) Shh+/− placentas infected with either control-shRNA- or Gli3-shRNA-expressing lentiviruses. Placentas were subjected to serial paraffin-embedded sections and were stained for the main components of Shh pathway by immunohistochemistry. The quantitative histomorphometry was performed by using the Osteomeasure Analysis System, the immunohistochemistry signals from respective control placentas were defined as 1. *P<0.05 versus respective controls
