Figure 5
From: Sonic hedgehog through Gli2 and Gli3 is required for the proper development of placental labyrinth
Histological analysis of placentas after genetic modifications. (a−b’, e−f’ and i−j’) H&E staining of placentas after genetic modifications. (c−d’, g−h’ and k−l’) Immunohistochemistry staining for cytokeratin (CK) and laminin in placentas. CK marks maternal blood lacunae (MBL) and laminin defines fetal blood vessel (FBV) spaces. (m−r) Relative areas of MBL and FBV spaces. Scale bar: 50 μm. The relative areas arisen from Shh−/− placentas were defined as 100%, *P<0.05 versus respective controls
