Figure 6
From: Sonic hedgehog through Gli2 and Gli3 is required for the proper development of placental labyrinth
Quantitative RT-PCR assays for the mRNA levels of trophoblast lineage differential markers of placentas (E12.0) after genetic modifications. (a–e) The mRNA levels of PL-1, Tpbpa, and PECAM-1 were determined in the placentas after various genetic modifications. (b–f) The mRNA levels of SynA, SynB, Ctsq, and Gcm1 were determined in the placentas after various genetic modifications. PL-1, Tpbpa, PECAM-1, SynA, SynB, Ctsq, and Gcm1 were the respective markers for trophoblast gaint cells, spongiotrophoblasts, endothelial cells, layer I syncytiotrophoblasts, layer II syncytiotrophoblasts, sinusoidal trophoblast gaint cells, and syncytiotrophoblasts. The relative mRNA levels arisen from respective controls were defined as 1, *P<0.05; †P<0.05 versus respective controls
