Figure 1 | Cell Death & Disease

Figure 1

From: Endoplasmic reticulum stress-regulated CXCR3 pathway mediates inflammation and neuronal injury in acute glaucoma

Figure 1

Ischemia-reperfusion induces the expression of CXCR3 and its ligands in retina. Ischemia-reperfusion (IR) was induced in wild-type (WT) mice and retinas were collected at 3, 6, 12, and 24 h after IR. (a) CXCL10 mRNA was determined by quantitative RT-PCR (qPCR). (b) CXCL10 protein expression was analyzed by ELISA. (c) In situ hybridization of CXCL10 mRNA. Retinal frozen sections from control and IR-performed mice at 6 h after IR were hybridized with a probe against mouse CXCL10 and detected with RNAscope Fluorescent Multiplex Kit. Green fluorescent signal reflects CXCL10 mRNA expression and DAPI (blue) stains nuclei. Arrows indicate CXCL10-expressed retinal ganglion cells. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer. (d and e) The mRNA levels of CXCL4 and CXCR3 were determined by qPCR. (f) Retinal frozen sections from control and IR-performed mice at 6 h after IR were incubated with an antibody against CXCR3. Fluorescent signal (red) reflects CXCR3 staining. *P<0.05 compared with control. N=3–4

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