Figure 2 | Cell Death & Disease

Figure 2

From: Endoplasmic reticulum stress-regulated CXCR3 pathway mediates inflammation and neuronal injury in acute glaucoma

Figure 2

CXCR3 deletion reduces retinal inflammation as well as oxidative and nitrosative stress in retina after IR. Ischemia-reperfusion was induced in WT and CXCR3 ko mice and retinas were collected at 24 h after IR. (a) Retina was stained with isolectin B4 (red, for vessels and activated microglia/monocyte) and IbaI antibody (green, for microglia/monocyte). Arrows show microglia/monocyte with different morphology suggesting different activation status. (b) The mRNA level of CD11b in retinas was analyzed by qPCR. (c) WT mice received bone marrow transplant from GFP mice and 6 weeks later they were subjected to IR. Retinas were fixed and labeled for IbaI (red) at 24 h after IR. (d and e) qPCR analysis of IL-1β and E-selectin mRNA expression in retina at 24 h after IR. (f) Nitrotyrosine level in retina was analyzed by western blot at 24 h after IR. Actin was used as an internal loading control. *P<0.05 compared with control; #P<0.05 compared with WT-IR. N=3–4

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