Figure 4
ER stress is involved in CXCL10 expression. (a–c) WT mice were exposed to IR and retinas were collected at 3 and 24 h after injury. The expression of GRP78, CHOP, and ATF4 mRNA was analyzed by qPCR. *P<0.05 compared with control. (d) WT mice were injected intraperitoneally with ER stress inhibitors, 4-phenylbutyric acid (PBA) or tauroursodeoxycholic acid (TUDCA) at 1 h before IR injury. Retinas were collected 6 h later and CXCL10 mRNA expression was assessed by qRT-PCR. *P<0.05 compared with sham control; #P<0.05 compared with vehicle-treated IR. (e–g) Twenty-four hours after WT and CXCR3 ko mice were exposed to IR injury, retina mRNA was extracted to analyze ER stress target genes (GRP78, CHOP and ATF4) by qPCR. *P<0.05 versus control; ns: not significant compared with WT-IR. N=4
