Figure 3
sc-tPA-promoted neuronal calcium influx is dependent on its interaction with NMDARs. Calcium video imaging performed on cortical neurons. (a) After two control NMDA stimulations used as baseline, neurons were incubated for 45 min in the presence of either buffer (control, n=104 cells), sc-tPA at 300 nM (sc-tPA n=111 cells) or GluN1 antibody at 10 μg/ml (n=99 cells) alone or in combination (sc-tPA+GluN1 antibody; n=109 cells) prior to a second set of NMDA stimulations. Percentages of potentiation or inhibition after treatment are calculated for each cell. (b) Percentages of potentiation or inhibition after treatment are calculated for each cell and reported as percentages of responsiveness for each group. (c) In the same protocol, neurons were incubated for 45 min in the presence of buffer (control, n=75 cells), sc-tPA at 300 nM (sc-tPA, n=77 cells) or AG1478 at 5 μM (AG1478, n=77 cells) alone or in combination (sc-tPA+AG1478, n=90 cells) prior to a second set of NMDA stimulations (2 × 25 μM, 30 s). (d) Percentages of responsiveness for each group (mean±S.E.M.; *P<0.0001 Kruskal–Wallis test followed by Mann–Whitney test; #P<0.0001 Wilcoxon test comparison of preincubation and postincubation responses). NS: not significant
