Figure 4
AG1478 reverses the inhibitory effect of tc-tPA on NMDAR signaling independently of its interaction with NMDARs. (a) After two NMDA stimulations used as baseline, neurons were incubated for 45 min in the presence of buffer (control, n=74 cells), AG1478 at 5 μM (AG1478, n=84 cells) or tc-tPA at 300 nM alone or in combination (tc-tPA, n=70 cells; tc-tPA+AG1478, n=81 cells) prior to a second set of NMDA stimulations. Percentages of potentiation or inhibition after incubation are calculated for each cell. (b) Percentages of potentiation or inhibition after incubation are calculated for each individual cell and reported as percentages of responsiveness for each group. (c) In the same protocol, neurons were incubated for 45 min in the presence of buffer (control, n=99 cells), GluN1 antibody at 10 μg/ml (GluN1 antibody, n=99 cells) or tc-tPA at 300 nM either alone or in combination tc-tPA 300 nM n=103 cells, tc-tPA+GluN1 antibody n=106 cells) prior to a second set of NMDA stimulations (2 × 25 μM, 30 s). (d) Percentages of potentiation or inhibition after incubation are calculated for each individual cell and reported as percentages of responsiveness for each group (mean±S.E.M.; *P<0.0001, Kruskal–Wallis test followed by Mann–Whitney test; #P<0.0001 Wilcoxon test of comparison of preincubation and postincubation responses). NS: not significant
