Figure 5
Only high concentrations of active sc-tPA promote excitotoxicity. (a) Cortical neurons (12-13 DIV) were exposed for 1 h to NMDA (50 μM) in the presence of sc-tPA or tc-tPA. Neuronal death was quantified 24 h later. (b and c) Same experiments as in panel (a) were performed with neurons exposed for 24 h to NMDA (10 μM) in the presence of sc-tPA alone or in combination with tPA stop (10 nM, b) or GluN1 antibody (10 μg/ml, c). (mean±S.E.M.; n=3 experiments; 4 wells per condition; *P<0.05, NS: not significant, Kruskal–Wallis test followed by Mann–Whitney test)
