Figure 1

Hipk2 expression in hippocampus, cortex, and striatum of wild-type mice. RNA extracted from hippocampus (A), cortex (B), and striatum (C) of wild-type mice at different ages was analyzed by qRT-PCR for Hipk2 expression. The G6pd expression level was used for normalization. Two-way repeated-measures ANOVA P<0.001; *P<0.001 versus p1, **P<0.001 versus all time points, n=3 for each group of age. HIPK2 expression in different brain regions (D). Double labeling of HIPK2 and Hoechst in hippocampus (D a–f), cortex (D g–l), and striatum (D m–r) by confocal microscopy analysis. Scale bar, 200 μm. High magnification of a portion of hippocampus (D d–f), cortex (D j–l), and striatum (D p–r). Scale bar, 20 μm