Figure 1 | Cell Death & Disease

Figure 1

From: Activation of surrogate death receptor signaling triggers peroxynitrite-dependent execution of cisplatin-resistant cancer cells

Figure 1

Sensitivity of A549 WT, R1 and R2 cells to platinum-based drugs. Cells were treated with (a) cisplatin (μM) or (b) carboplatin (mM) at the indicated doses for 24 h and cell viability was assessed by MTT assay. P<0.01 compared with WT treated at respective dose. (c) WT, R1 and R2 cells were treated with various concentrations of cisplatin for 24, 48 and 72 h. Cell viability was determined by MTT assay and expressed as % of untreated control. (d) Cells were treated with increasing doses of cisplatin for 24 h and DNA content was analyzed by flow cytometry after staining with propidium iodide (PI); y axis: events, x axis: PI linear fluorescence, n=3. (e) WT, R1 and R2 cells were treated with the indicated doses of cisplatin for 24 h and caspase 3, 8 or 9 processing and PARP cleavage was determined by western blotting. Data shown are mean±S.D. of at least three independent experiments. In panels a and b: ** and ## indicate P-value<0.005 compared to cisplatin sensitivity of WT cells. In panel c: * and ** indicate P-value<0.05 compared to WT cells treated with cisplatin for 48 and 72h, respectively

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