Figure 6 | Cell Death & Disease

Figure 6

From: Activation of surrogate death receptor signaling triggers peroxynitrite-dependent execution of cisplatin-resistant cancer cells

Figure 6

TRAIL exposure restores apoptosis sensitivity of cisplatin-resistant (CR) cells. (a) A549 R1, (b) H2030 CR and (c) A2780 CR cells were pretreated with varying doses of TRAIL for 2 h and followed with the indicated doses of cisplatin for 24 h. Cell viability was assessed by MTT and expressed as % of untreated control cells. Data shown are mean±S.D. of at least three independent experiments. Concurrently, the cell lysates from (d) A549 R1, (e) H2030 CR and (f) A2780 CR were collected to assay for caspase 3 and caspase 8 processing as well as PARP cleavage by western blot analysis. GAPDH was used as loading control. *, #, Δ indicated P-Value<0.05 compared to cells treated with cisplatin alone

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