Figure 3 | Cell Death & Disease

Figure 3

From: Protective effect of autophagy on human retinal pigment epithelial cells against lipofuscin fluorophore A2E: implications for age-related macular degeneration

Figure 3

3-MA inhibited A2E-induced autophagy in RPE cells. (a) Fluorescence microscopy was used to detect the formation of LC3 puncta in RPE cells treated with 25 μM A2E and/or 10 mM 3-MA for 12 h. Scale bar: 20 μm. (b) Quantification of LC3-positive puncta per cell. Data are shown as the means±S.E.M., n=6. **P<0.01, ***P<0.001, ANOVA. (c) Western blot analysis for protein expression of Beclin-1, LC3-I, and LC3-II in RPE cells treated with 25 μM A2E or A2E (25 μM) combined with 10 mM 3-MA for 12 h. (d and e) Quantification of expression levels of Beclin-1 and LC3-II proteins. Data are presented as the means±S.E.M., n=3. **P<0.01, ***P<0.001, ANOVA

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