Figure 4 | Cell Death & Disease

Figure 4

From: Protective effect of autophagy on human retinal pigment epithelial cells against lipofuscin fluorophore A2E: implications for age-related macular degeneration

Figure 4

3-MA and A2E together exacerbated RPE cells death and stimulated cytokine production. Cells were exposed to 25 μM A2E and/or 10 mM 3-MA for 12 h. (a) Typical TEM photomicrographs of RPE cells treated with A2E or A2E combined with 3-MA. Scale bar: 500 nm. (b) Cell viability was determined with the CCK-8 assay. Data are presented as the means±S.E.M., n=6. **P<0.01, ***P<0.001, ANOVA. (c and d) Protein expression of inflammatory factors and angiogenic cytokines were detected by multiplex cytokine assay in RPE cells treated with A2E or A2E combined with 3-MA. Data are presented as the means±S.E.M., n=3. *P<0.05, **P<0.01, ***P<0.001, ANOVA

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