Figure 6 | Cell Death & Disease

Figure 6

From: Protective effect of autophagy on human retinal pigment epithelial cells against lipofuscin fluorophore A2E: implications for age-related macular degeneration

Figure 6

Rapamycin restored cell viability and cytokine production to normal levels in RPE cells incubated with A2E. RPE cells were treated with 25 μM A2E and/or 100 nM rapamycin for 12 h. (a) Representative TEM photomicrographs of RPE cells treated with A2E or A2E combined with rapamycin. Scale bar: 500 nm. (b) Cell viability was probed by the CCK-8 assay. Data are presented as the means±S.E.M., n=6. **P<0.01, ***P<0.001, ANOVA. (c and d) Protein expression of inflammation factors and an angiogenic cytokine was detected via a multiplex cytokine assay in RPE cells treated with A2E or A2E combined with rapamycin. Data are presented as the means±S.E.M., n=3. *P<0.05, **P<0.01, ***P<0.001, ANOVA

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