Figure 1 | Cell Death & Disease

Figure 1

From: Membrane-bound and soluble Fas ligands have opposite functions in photoreceptor cell death following separation from the retinal pigment epithelium

Figure 1

FasL mediates photoreceptor cell death induced by retinal detachment. (a) Time course of TUNEL-positive cell density in outer nuclear layer (ONL) in BALB/c WT and BALB/c FasL−/− mice (n=6 each group and time point). The peak of TUNEL-positive cell density was day 1 in both groups. BALB/c FasL−/− mice showed significantly less photoreceptor cell death than BALB/c WT mice 1 day after retinal detachments (**P<0.01). (b) TUNEL (green) and TO-PRO-3 (blue) staining at 24 h after retinal detachment. Only ONL showed TUNEL-positive cells in both BALB/c WT and BALB/c FasL−/− mice. (c) ONL/INL (inner nuclear layer) ratio 7 days after retinal detachment (n=6 each). ONL/INL ratio was significantly higher in BALB/c FasL−/− than in BALB/c WT mice (*P<0.05). (d) Time course of TUNEL-positive cell density in ONL in B6129 WT and B6129 ΔCS mice (n=6 each group and time point). The peak of TUNEL-positive cell density was on day 1 in both groups. B6129 ΔCS mice showed significantly more photoreceptor cell death than B6129 WT mice at 24 h (*P<0.05), 120 h (*P<0.05), and 168 h (*P<0.001) after retinal detachment. (e) TUNEL (green) and TO-PRO-3 (blue) staining at 24 h after retinal detachment. Only ONL showed TUNEL-positive cells in both B6129 WT and B6129 ΔCS mice. (f) ONL/INL ratio 7 days after retinal detachment (n=6 each). ONL/INL ratio was significantly lower in B6129 ΔCS than in B6129 WT mice (*P<0.05). Scale bar, 50 μm. The graphs show mean±S.E.M. GCL, ganglion cell layer; UT, untreated

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