Figure 4 | Cell Death & Disease

Figure 4

From: Membrane-bound and soluble Fas ligands have opposite functions in photoreceptor cell death following separation from the retinal pigment epithelium

Figure 4

Classically and alternatively activated subretinal macrophages after induction of retinal detachment. (a–c) Ccr2, Ly6c, and Cx3cr1 mRNAs expression in the subretinal space was evaluated by LCM followed by qPCR using BALB/c WT and BALB/c FasL−/− mice at 1 and 7 days after retinal detachment (n=6 each group and time point). Ccr2 mRNA was significantly higher in day 1 than in day 7 in each group (WT: **P<0.01, FasL−/−: *P<0.05) (a). Ly6c mRNA was significantly higher in day 1 as compared with day 7 (WT: *P<0.05, FasL−/−: **P<0.01) (b). On the other hand, Cx3cr1 mRNA was significantly higher in day 7 than in day 1 (WT: ***P<0.001, FasL−/−: ***P<0.001) (c). There were no significant differences in the three mRNAs expression between BALB/c WT and BALB/c FasL−/− mice. (d–f) Ccr2, Ly6c, and Cx3cr1 mRNAs expression in the subretinal space in B6129 WT and B6129 ΔCS mice at 1 and 7 days after retinal detachment (n=6 each group and time point). Ccr2 mRNA tended to be higher in day 1 than in day 7 in each group; however, the differences did not achieve statistical significance (d). Ly6c mRNA was significantly higher in day 1 as compared with day 7 in B6129 ΔCS mice (**P<0.01). B6129 WT mice showed higher Ly6c mRNA in day 1 than in day 7; however, the difference did not reach statistical significance (e). Cx3cr1 mRNA was significantly higher in day 7 than in day 1 (WT: *P<0.05, ΔCS: ***P<0.001) (f). There were no significant differences in the three mRNAs expression between B6129 WT and B6129 ΔCS mice. The graphs show mean±S.E.M.

Back to article page