Figure 2 | Cell Death & Disease

Figure 2

From: Apoptotic cell clearance of Leishmania major-infected neutrophils by dendritic cells inhibits CD8+ T-cell priming in vitro by Mer tyrosine kinase-dependent signaling

Figure 2

Activation markers on DCs following capture of infected and uninfected neutrophils. (a) Representative dot plots of DCs cultured or not with eGFPhiRFP− or eGFPhiRFP+ dermal neutrophils for 12 h and analyzed for expression of CD11c and GFP signal. Gates represent populations of CD11c+ and CD11c+GFP+ cells. (b) Representative histogram plots of CD11c+ and CD11c+GFP+ gated cells stained for MHC II, CD40, CD86 and CD80. Data are representative of three independent experiments. (c) Mean fluorescence intensity (MFI) of MHC II, CD40, CD86 and CD80 expression on CD11c+ DC (white bars), CD11c+GFP+RFP− (gray bars) and CD11c+GFP+RFP+ (black bars) gated cells. Mean MFI±1 S.D. calculated from three independent experiments

Back to article page