Figure 1 | Cell Death & Disease

Figure 1

From: Mitochondrial protection impairs BET bromodomain inhibitor-mediated cell death and provides rationale for combination therapeutic strategies

Figure 1

I-BET762 induces apoptosis in mouse and human models of B-cell lymphoma. (a and b) Eμ-myc, RAJI, RL and U2932 B-cell lymphomas were treated with respective LD70 concentrations of I-BET762 for 48 or 240 h respectively. Apoptosis was assessed by flow cytometry analysis of TMRE, surface exposure of phosphotidylserine (annexin V staining) and cell-cycle analysis. Each point represents the mean value ±S.E. of three individual experiments, *P<0.05. (c) Western blot analysis on whole-cell lysates prepared from mouse and human lymphoma cell lines treated, respectively, for 24 and 72 h with corresponding LD70 concentrations of I-BET762 or DMSO. The protein expression of BRD4 and Myc was assessed. GAPDH was used as a loading control

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