Figure 1 | Cell Death & Disease

Figure 1

From: Synergistic killing of human small cell lung cancer cells by the Bcl-2-inositol 1,4,5-trisphosphate receptor disruptor BIRD-2 and the BH3-mimetic ABT-263

Figure 1

Cell death induction by BIRD-2. (a) IC50s of BIRD-2 and Scr control peptide (scrambled version of BIRD-2) in SCLC lines treated for 48 h with a broad range of peptide concentrations. Full dose–response curves were repeated at least three times. (b) Representative dose–response curves of SCLC lines treated for 48 h with BIRD-2. (c) Representative dose–response curves comparing H2171 cells treated for 48 h with BIRD-2 or Scr. (d and e) Time course of cell death induction by BIRD-2 in SCLC lines treated with 15 μM (H2171) or 20 μM (H1092) BIRD-2 or Scr at corresponding concentrations. (f) Dose–response curves comparing BIRD-2 sensitivity in SCLC cells (S), NSCLC cells (NS), and normal lung epithelial cells (N), each treated for 24 h with BIRD-2 at the indicated concentrations. (g) Graph based on data in Supplementary Table S1 comparing IC50s for BIRD-2 and ABT-263; each symbol represents an individual SCLC line. The horizontal line corresponds to an ABT-263 IC50 of 1 μM. (h) H2171 cells were treated for 48 h with variable concentrations of BIRD-2, ABT-263, or 127 : 1 BIRD-2+ABT-263 (BIRD-2 IC50 to ABT-263 IC50 ratio of 127 : 1). Cell viability was measured by CTG assay

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