Figure 1 | Cell Death & Disease

Figure 1

From: Affinity purification-mass spectrometry analysis of bcl-2 interactome identified SLIRP as a novel interacting protein

Figure 1

Functional analysis of bcl-2-interacting proteins. (a) Immunocomplexes (IMs) separation by SDS-PAGE. H1299 control (H1299 FLAG) and bcl-2-overexpressing (H1299 FLAG-bcl-2) cells were immunoprecipitated with anti-FLAG antibody. IMs were recovered, separated by SDS-PAGE and visualized by Coomassie staining. Lines were cut in 12 pieces, digested by trypsin and analyzed by nLC-MS/MS. Asterisk on the image indicates the band running at ~12 kDa in which SLIRP was identified. (b) Functional analysis by PANTHER classification system. Pie charts showing protein classification by cellular component, organelle, biological process and molecular function. The percentage of annotated categories is reported

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