Figure 2 | Cell Death & Disease

Figure 2

From: Affinity purification-mass spectrometry analysis of bcl-2 interactome identified SLIRP as a novel interacting protein

Figure 2

In vitro validation of bcl-2/SLIRP interaction. (a) Bcl-2 and SLIRP co-immunoprecipitation in H1299 control (H1299 FLAG) and bcl-2-overexpressing (H1299 FLAG bcl-2) cells. Western blot analysis of FLAG-bcl-2 and SLIRP proteins from immunocomplexes (IMs) obtained using anti-FLAG or anti-SLIRP antibodies. (b) Bcl-2 and SLIRP co-immunoprecipitation in MDA-MB 231 control (231 FLAG) and bcl-2-overexpressing (231 FLAG-bcl-2) cells. Western blot analysis of FLAG-bcl-2 and SLIRP proteins from IM obtained using anti-FLAG antibody. (c) Bcl-2 and SLIRP co-immunoprecipitation in HL60 and HeLa cell lines. Western blot analysis of bcl-2 and SLIRP proteins from IM obtained in HL60 cells using endogenous anti-bcl-2 antibody and in HeLa cells using endogenous anti-SLIRP antibody. (a, c) Normal rabbit serum was used as control. (c) Normal mouse serum (NMS) was used as control

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