Figure 4 | Cell Death & Disease

Figure 4

From: Targeting RING domains of Mdm2–MdmX E3 complex activates apoptotic arm of the p53 pathway in leukemia/lymphoma cells

Figure 4

MMRis activate the p53 pathway in cancer cell lines. (a) p53 protein accumulation in MMRi-treated HCT-8 cells. HCT-8 cells were treated with indicated compounds (5 μM) for 8 h and the whole-cell lysates were analyzed by WB of p53 with tubulin as a loading control. (b) Time and concentration-dependent induction of p53 and Mdm2 accumulation by MMRi64 in HCT-8 cells. HCT-8 cells were treated at the indicated concentrations of MMRi64 (right) for 8 h and for the indicated time at 5 μM (left) and whole-cell lysates were analyzed by WB of HdmX, Hdm2 and p53. Tubulin was used for loading control. (c) Activation of the p53 pathway by MMRi64 in NALM6 cells. NALM6 cells were treated for indicated time at the indicated concentrations of MMRi64 or Nutlin3a and the whole-cell lysates were analyzed by WB for p53, Hdm2, HdmX and tubulin

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