Figure 6
MMRi64 lymphoma cell growth via p53-dependent and p53-independent mechanisms. (a) Effects of p53 status on MMRi64-induced growth inhibition in Emu-myc lymphoma cells. The mouse Emu-myc lymphoma cells of different p53 status were cultured in the presence of 0.5 and 1 μM of MMRi64 and the numbers of viable cells were counted by Trypan blue exclusion at 72 h of treatment and plotted in histograms. (b) Effect of p53 status on Nutlin3a and MMRi64 sensitivity in colon cancer cells. HCT116 and HCT116-p53-/- cells were treated with indicated concentrations of drugs for 72 h and drug-induced growth inhibition was measured by MTT method and plotted in histograms. (c) Effect of MMRi64-Nutlin3a combination on expression of p53, Mdm2, MdmX and apoptotic cleavage of PARP in NALM6 cells. WB analysis of p53, Mdm2, MdmX and PARP cleavage after NALM6 cells were treated with Nutlin3a (1 μM) or MMRi64 (1 μM) alone or in combination for 8 and 24 h. (d) Flow cytometric analysis of NALM6 cells treated with indicated concentrations of Nutlin3a or MMRi64 alone or in combination for 48 h. Cells were fixed and stained with PI and subjected to flow cytometric analysis. The data were from triplicates of a single experiment with standard deviations within 0.06–0.5%. (e) Histograms of sub-G1 populations induced by MMRi64 or Nutlin3a alone or in their combinations at the indicated concentrations
