Figure 5 | Cell Death & Disease

Figure 5

From: Cellular prion protein promotes post-ischemic neuronal survival, angioneurogenesis and enhances neural progenitor cell homing via proteasome inhibition

Figure 5

Proteasome-mediated HIF-1α stabilization initiates PrPc-induced neuroprotection. (a) Proteasomal activity analyzed by Suc-LLVY-AMC cleavage, (b) oxidative stress determined by thiobarbituric acid reactive substances (TBARS) analysis, (c) HIF-1α and PACAP-38 abundance evaluated by western blotting and (d) infarct volume assessed by TTC staining of WT, PrP−/− and PrP+/+ mice exposed to 45 min MCA occlusion followed by 24 h reperfusion. For studies in (a–c), tissue samples were obtained from left ischemic hemispheres, and western blots in (c) were normalized with optic densities measured in β-actin blots (representative western blots are also shown). For studies in (d), animals had intrastriatally been pretreated with the solvent DMSO (control) or with the proteasome inhibitor BSc2118 12 h before MCA occlusion. *Significantly different from WT mice (a–c) or from corresponding control mice (d), P<0.05. #Significantly different from PrP−/− or PrP+/+ mice, P<0.05

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