Figure 2 | Cell Death & Disease

Figure 2

From: Intrinsic and chemo-sensitizing activity of SMAC-mimetics on high-risk childhood acute lymphoblastic leukemia

Figure 2

TNF-α signaling upon BV6 exposure. Western blot analysis of (a) cIAP1 degradation, (b) NIK accumulation and (c) NF-κB activation in BCP-ALL cell lines exposed to BV6 for indicated time points (UoCB6 and REH: 1 μM BV6; Nalm-6 and RS4;11: 10 μM BV6; or equivalent doses of DMSO for 24 h). For each analysis, one representative blot of three is shown. Arrows indicate NIK. (d) Increased TNF-α protein levels in cellular supernatants of BCP-ALL cell lines treated with BV6 or control as indicated (mean and S.D. of three independent experiments each performed in duplicate). (e–h) TNFR complex II formation upon BV6 exposure (UoCB6 and REH: 1 μM; Nalm-6 and RS4;11: 1 μM (+) or 10 μM (++) BV6) and dependency on TNF-α (with or without 40 μg/ml etanercept). Caspase-8 immunoprecipitation in the presence of 10 μM zVAD.fmk (UoCB6, REH and Nalm-6), and western blot analysis of indicated proteins (one representative experiment of three is shown)

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