Figure 3 | Cell Death & Disease

Figure 3

From: Intrinsic and chemo-sensitizing activity of SMAC-mimetics on high-risk childhood acute lymphoblastic leukemia

Figure 3

Apoptosis signaling upon BV6 treatment. (a) Loss of mitochondrial membrane potential (MMP) in BCP-ALL cell lines upon BV6 treatment (UoCB6 and REH: 1μM; Nalm-6 and RS4;11: 10 μM BV6; 24 h, with or without 40 μg/ml etanercept; mean and S.D. of three independent experiments each performed in triplicate, significance by Mann-Whitney U-test, *P<0.01. (b) Caspase-3 activation in BCP-ALL cell lines upon BV6 treatment (UoCB6 and REH: 1 μM; Nalm-6 and RS4;11: 1 μM (+) or 10 μM (++) BV6; 24 h), and (c) TNF-α dependency of the BV6-sensitive leukemia cell lines UoCB6 and REH (additional treatment with or without 40 μg/ml etanercept), western blot analysis. (d) PARP cleavage upon BV6 treatment for indicated time points (UoCB6 and REH: 1 μM; Nalm-6 and RS4;11: 10 μM BV6), western blot analysis. For each western blot analysis, one representative experiment out of three is displayed

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