Figure 5
BV6-induced cell death in primary ALL. Cell death induction in primary, patient-derived BCP-ALL samples (n=40) by BV6 (A) and dependency on TNF-α (n=20 primografts; B). Cell death by flow cytometry according to forward and side scatter criteria upon treatment with 1 μM BV6 for 48 h and with 40 μg/ml (BV6+Et) or without, BV6-induced cell death was calculated as the difference of total and spontaneous cell death; experiments performed in triplicate; mean and S.D. are indicated. (C) Activation of caspase-3 (a), and cytochrome c release (b) upon BV6 treatment (1 μM, 18 h) in primograft samples. Percentages of cells with activated caspase-3 and cells with released cytochrome c were estimated by flow cytometry in triplicate, bars represent mean values and S.D.
