Figure 2
miR-302 promotes the proliferation of pluripotent and adult stem cells. (a) Crystal violet staining assessed the cell growth in different miR-302s antagomir concentration-treated hNT-2 cells. miR-NC antagomir was used as negative control. (b) The growth curve at different time points was obtained by the cell counting Kit-8. Data are presented as mean±S.D. (n=8). (c) Cell proliferation was compared by BrdU incorporation rates as revealed by immunocytochemical staining in miR-302s-downregulated hNT-2. Scale bars, 100 μm. (d) Quantitative analysis of immunocytochemical staining for BrdU positive cells. BrdU positive cells were counted under a high power field and normalized by the total cell number. (e) The cell growth in miR-302s-upregulated hMSCs was assessed by crystal violet staining. miR-302s: miR-302-upregulated hMSCs by a miR-302-overexpressing lentiviral vector (miR-302a, miR-302b, miR-302c and miR-302d in combination), EGFP: a scrambled sequence infected hMSCs. (f) Immunocytochemical staining was used to detect the incorporation rates of BrdU in miR-302s-overexpressed hMSCs. Scale bars, 100 μm. (g) The growth curve of miR-302s-overexpressed hMSCs at different time points was obtained by the cell counting Kit-8 assay. Lentivector expressing a scrambled sequence was used as a control. (h) The expression level of the proliferative index PCNA was investigated in the xenografts using an immunohistochemical staining method. Scale bars, 100 μm. P-values were obtained using Student's t-test
