Figure 5 | Cell Death & Disease

Figure 5

From: Plasma membrane poration by opioid neuropeptides: a possible mechanism of pathological signal transduction

Figure 5

Pore size estimation. (a) Application of 1 μM Big Dyn at holding voltage of −100 mV elicited the appearance of progressively increasing basal current noise of HEK293 cells. (b) Indicated fragments of basal current recording from (a) are shown in milliseconds time scale. (c) All current surges under Big Dyn from the cell shown on (a) were used for estimation of event mode amplitude, resulting mode diameter of pore near 2.7 nm. The holding voltage was −100 mV, NMDG-HEPES solutions (see Materials and Methods) was used in this experiment to minimize errors connected with activities of basal ionic conductances

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