Figure 3
(a) HPV+ SiHa cells are resistant to TRAIL-induced apoptosis as compared with the human osteosarcoma cell line U2OS. Cells (2 × 104 cells per well) were seeded into a 96-well plate, allowed to incubate overnight, and then treated with the indicated concentration of TRAIL in the presence of cycloheximide (5 μg/ml). The viability of cells was measured after overnight incubation using the MTT assay; the viability of cells untreated with TRAIL was set at 100% for each group. (b) The indicated concentrations of three small molecules were added to SiHa cells, and an MTT assay was preformed after overnight incubation. The viability of cells untreated with small molecules was set as 100%. (c) The indicated concentrations of myricetin or spinacine were added 4 h prior to TRAIL (100 ng/ml) in the presence of cycloheximide (5 μg/ml) and incubated at 37 °C overnight. The viability of cells untreated with small molecules was set at 100%. (d and e) SiHa cells (2 × 104 cells per well) were seeded into a 96-well plate and allowed to incubate overnight. In all, 100 μM of myricetin and 50 μM of spinacine were added 4 h prior to either (d) doxorubicin or (e) cisplatin. Cell viability was measured after overnight incubation using the MTT assay, and the viability of cells untreated with either myricetin or spinacine was set at 100%. Experiments were performed in triplicate, and error bars indicate the S.D.
