Figure 5
Myricetin and spinacine (5) can re-sensitize HPV+, but not HPV−, cells to treatment with TRAIL. SiHa (2 × 104 cells per well) and C33A cells (1 × 104 cells per well) were seeded into a 96-well plate and allowed to incubate overnight, and then cells were pretreated in the presence or absence of (a) myricetin (100 μM) or (b) spinacine (50 μM) for 4 h. The indicated concentration of TRAIL along with cycloheximide (5 μg/ml) was added, and the cells were allowed to incubate overnight. Cell viability was measured by MTT assay, and the viability of cells untreated with TRAIL was set at 100%. Experiments were performed in triplicate, and error bars indicate the S.D.
