Figure 4
The activity of ATM is required for virus-induced autophagy and protection against CPT. (a) Caffeine (Caf), an inhibitor of ATM, decreases autophagy, as indicated by the decrease in the smaller LC3 band, in Dengue (Den)-infected cells (compare Den Caf with Den) after 24 h. (b) Caffeine (20 μM) also brings down the numbers of GFP puncta in infected HeLa-GFP-LC3 after 24 h. (c) Quantification of GFP-LC3 punctation from the micrographs (mean of 315 cells counted per sample). (d) Caffeine also reduces dengue-induced protection from CPT toxicity in MDCK cells 24 h after infection. (e) Another ATM inhibitor (KU55933 or ATMi) also reduces dengue-induced cell protection from CPT toxicity in MDCK cells by 24 h
