Figure 4

Pdcd4 deficiency promoted macrophage autophagy and attenuated lipid accumulation in plaques. apoe−/− and pdcd4−/−apoe−/− (DKO) mice were fed with a high-fat diet from 8 to 16 weeks to induce atherosclerotic lesions (apoe−/−: n=7; DKO: n=5). Autophagosome and LD were colocalized by double staining with anti-Lc3b antibody conjugated with Alexa 555 (red) and Bodipy (green) in aortic root (red, a). Macrophage and autophagosome were colocalizated by staining with anti-Moma-2 antibody conjugated with Alexa 488 (green) and anti-Lc3b antibody conjugated with Alexa 555 (red, b)