Figure 6

Pdcd4-mediated autophagy in bone marrow-derived cells contributed to the development of atherosclerosis. Eight-week-old female Ldl receptor-deficient (ldlr^−/−) mice were irradiated and transplanted bone marrow cells from WT or pdcd4^−/− mice (n=7 for each group). After bone marrow transplantation, mice were maintained under a pathogen-free condition and given a high-fat diet for 16 weeks to induce atherosclerosis. H&E staining was performed to show atherosclerotic lesions (a). Oil red O staining was applied to show aortic face lesions (b). Aortic sections were stained with anti-Moma-2 conjugated with Alexa 488 to demonstrate macrophage, and with anti-Lc3b conjugated with Alexa 555 to show autophagosome (c). **P<0.0